The PK136 monoclonal antibody reacts with mouse NK1.1 also known as CD161b/CD161c, KLRB1, NKR-P1A and Ly-55. NK1.1 is a type II integral membrane glycoprotein with a C-type lectin domain and is encoded by the Klrb1c/NKR-P1C gene. NK1.1 plays roles in NK cell activation and differentiation, IFN-γ production, cytotoxic granule release, and is thought to be involved in the generation of Th2 cells. NK1.1 is predominantly expressed as a disulfide-linked homodimer on NK cells however, it is also expressed on NK-T cells, a rare population of T lymphocytes. NK 1.1 is only expressed by C57BL/6, FVB/N, and NZB strains of mice and not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129 strains.

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Isotype?Mouse IgG2a, κ

Recommended Isotype Control(s)?InVivoMAb mouse IgG2a isotype control, unknown specificity

Recommended Dilution Buffer?InVivoPure pH 7.0 Dilution Buffer

Immunogen?Mouse spleen and bone marrow cells enriched for NK1+ cells

Reported Applications?in vivo NK cell depletion

Flow cytometry

Formulation?PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin?<2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity?>95%

Determined by SDS-PAGE

Sterility?0.2 μM filtered

Production?Purified from tissue culture supernatant in an animal free facility

Purification?Protein A

RRID?AB_1107737

Molecular Weight?150 kDa

Storage?The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

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PK136單克隆抗體與小鼠NK1.1反應(yīng)，也稱為CD161b / CD161c，KLRB1，NKR-P1A和Ly-55。NK1.1是一種具有C型凝集素結(jié)構(gòu)域的II型整合膜糖蛋白，由Klrb1c/NKR-P1C基因編碼。NK1.1在NK細胞活化和分化，IFN-γ產(chǎn)生，細胞毒性顆粒釋放中起作用，并被認(rèn)為參與Th2細胞的產(chǎn)生。NK1.1主要在NK細胞上表達為二硫鍵連接的同源二聚體，然而，它也在NK-T細胞上表達，NK-T細胞是一種罕見的T淋巴細胞群。NK 1.1僅由小鼠的C57BL / 6，F(xiàn)VB / N和NZB菌株表達，而不是AKR，BALB / c，CBA / J，C3H，DBA / 1，DBA / 2，NOD，SJL和129株表達。

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艾美捷InVivoMAb抗小鼠NK1.1#BE0036基本參數(shù)：

同種型?小鼠IgG2a， κ

推薦的同種型對照?體內(nèi)MAb小鼠IgG2a同種型對照，特異性未知

推薦的稀釋緩沖液?體內(nèi)純?pH 7.0 稀釋緩沖液

免疫原?富含NK1+細胞的小鼠脾臟和骨髓細胞

報告的應(yīng)用程序?體內(nèi)NK細胞去除

流式細胞術(shù)

配方?PBS，pH 7.0

不含穩(wěn)定劑或防腐劑

內(nèi)毒素?<2EU/mg （<0.002EU/μg）

通過LAL凝膠凝血測定法測定

純度?>95%

由?SDS-PAGE 決定

無?菌?0.2 μM 過濾

生產(chǎn)?從無動物設(shè)施中的組織培養(yǎng)上清液中純化

純化?蛋白A

里德?AB_1107737

分子量?150 千達

存儲?抗體溶液應(yīng)以4°C的儲備濃度儲存。 不要凍結(jié)。

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InVivoMAb抗小鼠NK1.1部分文獻引用：

in vivo NK cell depletion

Ghasemi, R., et al. (2016). "Selective targeting of IL-2 to NKG2D bearing cells for improved immunotherapy" Nat Commun 7: 12878.

in vivo NK cell depletion, Flow Cytometry

Maltez, V. I., et al. (2015). "Inflammasomes Coordinate Pyroptosis and Natural Killer Cell Cytotoxicity to Clear Infection by a Ubiquitous Environmental Bacterium" Immunity 43(5): 987-997.

in vivo NK cell depletion, Flow Cytometry

Wensveen, F. M., et al. (2015). "NK cells link obesity-induced adipose stress to inflammation and insulin resistance" Nat Immunol 16(4): 376-385.

in vivo NK cell depletion

Yamada, D. H., et al. (2015). "Suppression of Fcgamma-receptor-mediated antibody effector function during persistent viral infection" Immunity 42(2): 379-390.