12-HETE Hypertension ELISA背景：

This competitive ELISA kit is for determination of 12-HETE (12-Hydroxyeicosatetraenoic acid) levels in biological samples. The specificity of the 12-HETE ELISA was investigated using authentic 12-HETE and fatty acids which, based on their structure, might be anticipated to compete with 12-HETE for binding to antibodies against 12-HETE.

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HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxyl product produced from the reduction of 12(S)-hydroperoxytetraenoic eicosatetraenoic acid [12(S)-HpETE], which is itself is a 12-lipoxygenase metabolite of arachidonic acid. It has recently been reported that platelet 12(S)-HETE production is enhanced in the spontaneously hypertensive rat1. 12(S)-HETE levels and 12-lipoxygenase (12-LO) protein are increased in patients with essential hypertension2, also suggesting a role for this

metabolite in human hypertension. These metabolites exhibit a variety of biological activities such as mediation of angiotensin II–induced intracellular calcium transients in cultured rat vascular smooth muscle cells3 and as a second messenger in angiotensin-II induced aldosterone production4

. 12(S)-HETE also acts as an aggregator of polymorphonuclear leukocytes5 and is a highly selective ligand used to label mu opioid receptors6 and serves as a biomarker of Churg-Strauss syndrome7.

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Each kit can be used for triplicate analyses of up to 24 samples contains using a 96 well plate format, and contains a vial of 12-HETE standard, a vial of 12-HETE-conjugated horseradish peroxidase (HRP), and buffers for sample and HRP dilutions, and plate washing.

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這種競爭性ELISA試劑盒用于測定生物中12-HETE（12-羥基二十碳四烯酸）的水平樣品。使用真實的12-HETE和脂肪酸研究12-HETE ELISA的特異性可以預期在其結(jié)構(gòu)上與12-HETE競爭與針對12-HETE的抗體的結(jié)合?？?12-HETE僅與15-HETE和13-HODE輕微交叉反應。

HETE是花生四烯酸通過脂氧合酶代謝產(chǎn)生的副產(chǎn)物。

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12（S）-HETE是12-（S）-氫過氧四烯酸二十碳四烯酸還原產(chǎn)生的立體特異性羥基產(chǎn)物[12（S）-HpETE]，其本身是花生四烯酸的12-脂氧合酶代謝產(chǎn)物。最近有報道稱自發(fā)性高血壓大鼠血小板12（S）-HETE的產(chǎn)生增強1.12（S）-HETE水平和12-原發(fā)性高血壓患者脂氧合酶（12-LO）蛋白增加2，也暗示了這方面的作用人類高血壓的代謝產(chǎn)物。這些代謝產(chǎn)物表現(xiàn)出多種生物活性，如介導血管緊張素II誘導的培養(yǎng)大鼠血管平滑肌細胞內(nèi)鈣瞬變3作為第二個血管緊張素II誘導的醛固酮產(chǎn)生中的信使4.12（S）-HETE還充當多形核白細胞5是一種用于標記μ阿片受體的高選擇性配體6并作為丘格-斯特勞斯綜合征的生物標志物7.

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每個試劑盒可用于對多達24個樣品進行一式三份的分析，包含使用96孔板格式的樣品，并包含一個小瓶12-HETE標準，一小瓶12-HETE綴合的辣根過氧化物酶（HRP），以及用于樣品和HRP的緩沖液稀釋和板洗滌。

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艾美捷12-HETE Hypertension ELISA#DRD-12H1參考文獻：

1. Stern N, Kisch ES, Knoll E. Platelet lipoxygenase in spontaneously hypertensive rats. Hypertension. 1996;27:1149–1152.

2. González-Nú?ez, Daniel, Joan Claria, Francisca Rivera, Esteban Poch. Increased levels of 12(S)-HETE in patients with essential?hypertension. Hypertension 2001; 37: 334-338

3. Natarajan, Rama, Noe Gonzales, Linda Lanting, Jerry Nadler. Role of the Lipoxygenase pathway in?angiotensin II-induced vascular smooth muscle cell hypertrophy. Hypertension 1994; 23: I142

4. J L Nadler, R Natarajan and N Stern. Specific action of the lipoxygenase pathway in mediating angiotensin II-induced aldosterone?synthesis in isolated adrenal glomerulosa cells. J Clin Invest. 1987;80(6):1763–1769.

5. O'Flaherty JT, Thomas MJ, Lees CJ, McCall CE Neutrophil-aggregating activity of monohydroxyeicosatetraenoic acids.Am J Pathol. 1981 Jul;104(1):55-62.

6. Christie, M.J., C.W. Vaughan and S.L. Ingram. Opioids, NSAIDs, and 5-lipoxygenase inhibitors act synergistically in brain via?arachidonic acid metabolism. Inflamm. Res. 1999, 48: 1-4.

7. Szczeklik W, Sanak M, Mastalerz L, Soko?owska BM, Gielicz A, Soja J, Kumik J, Musia? J, Szczeklik A.12-hydroxy-eicosatetraenoic acid (12-HETE): a biomarker of Churg-Strauss syndrome. Clin Exp Allergy. 2012 Apr;42(4):513-22

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12-HETE Hypertension ELISA相關(guān)研究：

15-HETE Hypertension ELISA Kit?15H1