潮霉素B(Hygromycin B)是由吸水鏈霉菌產(chǎn)生的抗生素。潮霉素B是一種氨基糖苷類抗生素，通過抑制蛋白質(zhì)合成來殺死細(xì)菌，真菌和高等真核細(xì)胞。

溶解度SolubilityDMSO: 3.3 mg/ml PBS (pH 7.2): 3.3 mg/ml,溶于水

性狀：白色固體粉末

儲(chǔ)藏條件：Store desiccated at -20°C.

潮霉素B 的應(yīng)用：
潮霉素B 通常用于研究蛋白質(zhì)合成。潮霉素B 除抗細(xì)菌，真菌和高等真核細(xì)胞的功效外，潮霉素B 還是一種抗病毒劑，可在選擇性進(jìn)入因病毒感染而變成可滲透的細(xì)胞后抑制翻譯。它在基因?qū)嶒?yàn)中用作標(biāo)準(zhǔn)選擇抗生素，尤其是對(duì)于潮霉素抗性基因轉(zhuǎn)化細(xì)胞的選擇。

潮霉素B In vivo：
1.逐一添加每種溶劑：10％DMSO 40％PEG300 5％吐溫80 80％鹽水
溶解度：≥6.25 mg / mL（11.85 mM）; 澄清的溶液
2.逐一添加每種溶劑：10％DMSO 90％（鹽水中20％SBE-β-CD）
溶解度：≥6.25 mg / mL（11.85 mM）;?澄清的溶液
3.每種溶劑一添加：10％DMSO 90％玉米油
溶解度：≥6.25 mg / mL（11.85 mM）; 澄清的溶液.
為了保證產(chǎn)品使用質(zhì)量相關(guān)溶液，溶劑請(qǐng)找BIOFOUNT公司訂購。

案例：

a) 3000 synchronized L1 larvae were transferred to seeded 6 cm NGM plates and immediately treated with hygromycin B. Images of control and treated plates were acquired 40 hours later. b) Survival assay on hygromycin B dilution. 3000 synchronised L1 larvae were plated on seeded 6 cm plates and immediately treated with hygromycin B at the specified final concentrations. The plates were scored for L4 larvae after 40 hours and for adults after 90 hours. The experiment was performed in triplicate, error bars represent standard deviation. c) Selection of transgenic animals on hygromycin B. 10 synchronized L1 larvae carrying a hygromycin B resistance gene and expressing GFP/mCherry were mixed with the indicated number of wild type L1 larvae on a seeded 6 cm plate and immediately treated with hygromycin B (0.3 mg ml?1). The number of adult transgenic (fluorescence-positive) and non-transgenic (fluorescence-negative) animals was scored after 90 hours. The experiment was performed with two independent hygromycin B resistant transgenic strains.