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中英文說明丨抗原修復液(BTM-22030)方案

2023-07-05 16:01 作者:認真搞科研的科研達人  | 我要投稿

AntiFix??Universal Antigen?Retrieval Buffer, 10X

Catalog Number:?22030-50mL, 22030-500mL

Unit Size:?50 mL, 500 mL

Storage and Handling

Store at room temperature. Product is stable for at least 12 months from date of?receipt when stored as recommended. The buffer is non-toxic and non-flammable.

Product Description

AntiFix??Universal Antigen Retrieval Buffer is designed for heat-induced epitope?retrieval (HIER) of formalin-fixed, paraffin embedded (FFPE) tissues. The buffer?uses a proprietary reagent that catalyzes the reversal of formaldehyde cross-links?in FFPE tissue to restore antigen reactivity.

Fixation of tissue with formalin (formaldehyde) preserves tissue morphology, but?the cross-linking of proteins by formaldehyde can modify antibody binding sites or?epitopes so they are no longer detectable by immunohistochemistry. Traditionally,epitope reactivity is recovered by using protease treatment, or by heating slides in?either low pH citrate buffer or high pH Tris buffer. The optimal method for antigen?retrieval varies for different epitopes, requiring multiple buffers to be used for?different targets.

AntiFix??Universal Antigen Retrieval Buffer has been formulated to provide?excellent heat unmasking for a wide variety of targets at neutral pH, reducing the?need for buffer optimization. It has been validated in immunofluorescence staining?of FFPE tissue with our next-generation fluorescent CF? dyes using HRPmediated tyramide signal amplification.

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AntiFix Universal Antigen Retrieval Buffer可在中性pH條件下對多種靶標進行出色的解蔽。經(jīng)驗證,它可用于使用HRP介導的酪胺信號放大的下一代熒光CF?染料對FFPE組織進行熒光染色。50 mL 10 X緩沖液。

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艾美捷抗原修復液#BTM-22030特征:

中文名稱:抗脂多糖核心單克隆抗體(克隆號WN1 222-5) 現(xiàn)貨

英文名字:Lipopolysaccharide Core,?mAb WN1 222-5

Hycult編號:HM6011

規(guī)格:50ug

應用類型:FS、IA、P、WB

免疫原:小鼠來源的108sup> heat-killed bacteria

來源宿主:小鼠

反應性:抗脂多糖核心

保存建議:該產(chǎn)品為液體形式,推薦4℃保存,可保存1年。

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抗原修復液#BTM-22030相關研究:

編號?名稱

23007 TrueBlack⑧脂褐素自身熒光猝滅劑

23012 TrueBlack@IF背景抑制器系統(tǒng)(可滲透)

23013 TrueBlack@WB阻塞式Bufier套件

40083 NucSpo 470綠色核計數(shù)器

40061紅點TM2遠紅色核計數(shù)器

23001-

23002 EverBrite安裝介質(zhì)(帶或不帶DAPI)

23003-

23004 EverBrite W硬質(zhì)合金安裝介質(zhì)(帶或不帶DAPI)

23005 CoverGrip“Coverslip密封膠

22005 Mini Super“Pap Pen 2.5毫米筆尖,-400次使用

22006 Super“Pap Pen 4毫米筆尖,-800次使用

33000

33020 CF@染料或生物素Tyramide擴增試劑盒

22029 Tyramide擴增緩沖液+

92300-

92302混合正染色“”HRP抗體標記試劑盒

22023聚甲醛,4%PBS,即用型固定劑

22016滲透緩沖液

22017滲透和封閉緩沖液

22020 10X磷酸鹽緩沖鹽水(PBS)(4L Cubitainer@)

22013牛血清白蛋白組分V

22014牛血清白蛋白30%溶液

22010 10X魚膠封閉劑

22011魚膠粉

30015 DAB基板套件


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