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云端論腦預(yù)告 | 一作帶你讀Science

2020-12-12 14:01 作者:腦人言 0人讀過 | 我要投稿

現(xiàn)有的主流觀點認為PVH神經(jīng)元集群通過專線（labeled-line ?coding）編碼各種行為狀態(tài)：每種PVH神經(jīng)元類型都會承擔一種特定的功能，并僅在該特定的行為狀態(tài)下做出反應(yīng)。運用CaRMA成像，定量行為分析，機器學習等手段，我們發(fā)現(xiàn)PVH神經(jīng)元集群是通過不同的神經(jīng)元類型組合方式（grouped-ensemble coding）來編碼多種行為的，并揭示了神經(jīng)肽Y受體（Npy1r）能有效協(xié)同各種類型神經(jīng)元的活動。

論文題目：

文章摘要：

Brains encode behaviors using neurons amenable to systematic classification by gene expression. The contribution of molecular identity to neural coding is not understood because of the challenges involved with measuring neural dynamics and molecular information from the same cells. We developed CaRMA (calcium and RNA multiplexed activity) imaging based on recording in vivo single-neuron calcium dynamics followed by gene expression analysis. We simultaneously monitored activity in hundreds of neurons in mouse paraventricular hypothalamus (PVH). Combinations of cell-type marker genes had predictive power for neuronal responses across 11 behavioral states. The PVH uses combinatorial assemblies of molecularly defined neuron populations for grouped-ensemble coding of survival behaviors. The neuropeptide receptor neuropeptide Y receptor type 1 (Npy1r) amalgamated multiple cell types with similar responses. Our results show that molecularly defined neurons are important processing units for brain function.

報告嘉賓：

徐圣進，中科院神經(jīng)所博士。目前，在Janelia Research Campus的Scott Sternson研究組進行博后工作。結(jié)合單細胞RNA測序，深部腦區(qū)雙光子鈣成像和多重復(fù)用熒光原位雜交開發(fā)了CaRMA成像。運用CaRMA成像，定量行為分析，機器學習等手段研究了下丘腦室旁核（PVH）中多種類型的神經(jīng)元集群如何編碼多種生存行為，并系統(tǒng)地分析了下丘腦室旁核中基因表達、神經(jīng)元活動及動物行為之間的關(guān)系。

云端論腦——神經(jīng)科學青年學者云端論壇(Young Neuroscientist Zoom)，由中國科學院神經(jīng)所畢業(yè)生、加州大學博后郭延猛(UCSF)、馬韶蓉(UCSC)、周立(UCSF)于2020年四月發(fā)起，期待與全球青年神經(jīng)科學家拓寬視野，促進交叉研究開展。