中英文丨預激活APC-Cy7串聯(lián)說明書
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~780 nm.
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AAT Bioquest offers this preactivated APC-Cy7 to facilitate the APC-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
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別藻藍蛋白?(APC)?是一種從藍綠藻螺旋藻中分離出來的藻膽蛋白。與其他藻膽蛋白一樣，APC?是熒光的，具有J高的吸收率和高量子效率。它是一種可以通過常規(guī)蛋白質交聯(lián)技術輕松與抗體和其他蛋白質連接而不改變其光譜特性的蛋白質。APC-Cy7?是流式細胞術中常用的顏色。其主要吸收峰在?651 nm，發(fā)射峰在?~780 nm。
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艾美捷預激活APC-Cy7串聯(lián)?以促進?APC-Cy7?與抗體和其他蛋白質（如鏈霉親和素和其他二級試劑）的串聯(lián)偶聯(lián)。該預激活APC-Cy7串聯(lián)已準備好進行偶聯(lián)，與傳統(tǒng)繁瑣的基于?SMCC?的偶聯(lián)化學相比，其產量要高得多。
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艾美捷預激活APC-Cy7串聯(lián)普通儲備溶液制備：
將特定質量的ReadiUse? 預活化?APC-Cy7 Tandem復溶至給定濃度所需的適當溶劑體積。請注意，體積僅用于制備儲備溶液。
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艾美捷預激活APC-Cy7串聯(lián)組件：
A：ReadiUse? 預激活?APC-Cy7?串聯(lián)?1?瓶（1?毫克）
B：Buccutite??MTA?1?瓶?(100 μg)
C：旋轉脫鹽柱?不包含
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光譜特性：
消光系數(shù)（cm -1 M -1）?700000
激發(fā)（納米）?651
發(fā)射（納米）?779
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儲存、安全和處理：
H-短語?H303、H313、H333
危險符號?XN
有可能的使用?僅供研究使用?(RUO)
R-短語?R20、R21、R22
UNSPSC?12171501
