How to replace the sample needle on an ACQUITY Flow Through Needle (FTN) Sample Manager - WKB7319

如何更換穿針取樣管上的取樣針

OBJECTIVE or GOAL
目的或目標(biāo)
Replace the sample needle on an FTN.
更換 FTN 上的樣品針。

ENVIRONMENT
環(huán)境

• ACQUITY UPLC H-Class FTN Sample Manager H 級 FTN 樣品管理器

• ACQUITY UPLC I-Class FTN Sample Manager 一級 FTN 樣品管理器

PROCEDURE
程序

1. In the Console, select the Sample Manager-FTN from the system tree. 在控制臺中，從系統(tǒng)樹中選擇 Sample Manager-FTN

2. Click Maintain > Replace > Needle. 點(diǎn)擊維護(hù) > 更換 > 針頭

3. Open the sample compartment door. 打開樣品室的門

4. Using a T20 TORX driver, loosen the two captive screws that secure the access panel, and then remove the panel. 使用 T20 TORX 驅(qū)動程序，松開兩個圈養(yǎng)螺絲固定訪問面板，然后刪除面板

1. Remove the sample plate if a plate is loaded. 如果加載了樣板，則拆卸樣板

2. Unscrew the needle assembly's fitting from injector port 4. 從注射器口4擰下針頭總成的配件

1. Inside the sample compartment, open the needle-tubing guide cover, located on the roof of the compartment, by rotating clockwise. 在樣品室內(nèi)，打開位于樣品室頂部的針管導(dǎo)向蓋，順時針旋轉(zhuǎn)

1. Push the needle latch back to release the needle mounting cylinder from its mounting cavity, and the needle tubing from its notches. 推動針鎖存回來，釋放針安裝缸從其安裝腔，針管從其缺口

1. Lift the needle tip out of the needle guide at the bottom of the needle mechanism. 將針尖從針頭機(jī)構(gòu)底部的針頭導(dǎo)軌上提起

Warning: To avoid puncture wounds or damage to the end of the needle, do not touch or press the end of the sample needle.
警告: 為了避免穿刺傷口或針頭末端的損壞，請不要觸摸或按壓樣品針頭的末端。

1. Using a T10 TORX driver, loosen the screw on the needle-tubing clamp and remove the tubing. 使用 T10 TORX 驅(qū)動程序，松開針管夾上的螺絲，并刪除油管

1. Using a T6 TORX driver, loosen the needle guide set screw, and then remove the needle guide. 使用 T6 TORX 驅(qū)動程序，松開針引導(dǎo)設(shè)置螺絲，然后刪除針引導(dǎo)

1. Install the new needle guide and tighten the set screw. 安裝新的針頭導(dǎo)軌，擰緊固定螺絲

2. Holding the new needle assembly with both hands to control its position within the sample compartment, secure the mounting sleeve in the opening of the tubing clamp, with the fitting oriented toward the valve. Caution: To avoid letting go of the needle assembly and possibly damaging the tip of the needle, do not tighten the clamp screw during this step of the procedure. 用雙手握住新的針組件，以控制其在樣品室內(nèi)的位置，將安裝套管固定在油管夾的開口處，使安裝套管朝向閥門。注意: 為了避免松開針組件并可能損壞針頭的尖端，不要在此過程中擰緊夾緊螺絲

1. From inside the sample compartment, take hold of the needle assembly tubing as it enters the compartment from above. 從內(nèi)部的樣品隔間，抓住針裝配管，因?yàn)樗M(jìn)入隔間從上面

2. Ensure that the needle tubing is routed to the left side of the needle carriage's rails and is secured in the guide channel on the roof of the compartment. 確保針管路由到針車軌道的左側(cè)，并固定在隔間頂部的導(dǎo)向通道中

1. Remove the protective cap from the needle tip. 從針尖上取下保護(hù)帽

2. Insert the needle mounting cylinder into the mounting cavity. 將安裝針筒插入安裝腔內(nèi)

Caution: To avoid damaging the instrument, do not place the loose loop of tubing above the Z-flag.
警告: 為了避免損壞儀器，不要將松動的管路環(huán)置于 z 標(biāo)志之上。

1. Route the tubing through the two notches below the Z-flag. 將油管穿過 z 旗下的兩個凹槽

2. Push the needle latch forward to secure the needle assembly. 向前推針鎖存器以固定針總成

1. Using a T10 TORX driver, tighten the screw on the needle-tubing clamp. 使用 T10 TORX 驅(qū)動程序，擰緊針管夾上的螺絲

2. Insert the needle's tip into the needle guide at the bottom of the needle mechanism. 將針頭插入針頭機(jī)構(gòu)底部的針頭導(dǎo)軌中

1. Ensure that the needle tubing is fully inserted into port 4 on the injection valve, and then thread the fitting into the port, tightening the fitting securely. 確保針管完全插入注射閥的4口，然后將接頭螺紋固定在4口，緊固接頭

2. Reinstall the access panel using the T20 TORX driver to tighten the two screws that secure it to the front of the unit. 使用 T20 TORX 驅(qū)動程序重新安裝訪問面板，擰緊固定在機(jī)組前部的兩個螺絲

Requirement:?Ensure that the seal port tube is routed under the gap on the side of the access panel.
要求: 確保密封端口管路由在接入面板側(cè)面的間隙之下。

1. Close the sample compartment and fluidics compartment doors. 關(guān)閉樣品隔間和流體隔間門

2. Calibrate the needle's 校準(zhǔn)指針z?axis (see the ACQUITY UPLC online Help). 軸(請參閱 ACQUITY UPLC 在線幫助)

• See article: 參見文章:How to calibrate the needle Z axis - WKB28509 如何校準(zhǔn)針頭 z 軸 -WKB28509

1. Characterize the needle seal (see the ACQUITY UPLC online Help). 描述針密封(請參閱 ACQUITY UPLC 在線幫助)

2. Perform the needle seal readiness test (see the ACQUITY UPLC online Help). 執(zhí)行針密封準(zhǔn)備測試(請參閱 ACQUITY UPLC 在線幫助)

• See Video:

Diagnostics: Needle-Seal Readiness Testsite-436448.bcvp0rtal.com/detail/videos/how-to-series/video/4334162061001/diagnostics:-needle-seal-readiness