Enzymatic Reaction：

Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains (Brown and Wold 1973). The enzyme in excreted by the pancreas and takes part in the digestion of food proteins and other biological processes. Trypsin is a medium-sized globular protein and is produced as an inactive proenzyme, trypsinogen (Chen et al. 2009).

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Specificity:

Trypsin cleaves peptides on the C-terminal side of lysine and arginine amino acid residues. If a proline residue is on the carboxyl side of the cleavage site, the cleavage will not occur. If an acidic residue is on either side of the cleavage site, the rate of hydrolysis has been shown to be slower.

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applications:

Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase

Cell harvesting by?“trypsinization”

Mitochondria isolation

in vitro studies of proteins

Removing monolayers of cells from plastic and glass

Various hemagglutination procedures

Sample preparation for flow cytometric DNA analysis

Tryptic mapping

Fingerprinting and sequencing work

Environmental monitoring

Reduction of cell density in tissue culture

Subculturing cells

Cleavage fusion proteins

Generating glycopeptides from purified glycoproteins

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艾美捷Worthington胰蛋白酶特異性：

胰蛋白酶切割賴氨酸和精氨酸氨基酸殘基?C?端側(cè)的肽。如果脯氨酸殘基位于切割位點(diǎn)的羧基側(cè)，則不會(huì)發(fā)生切割。如果酸性殘基位于裂解位點(diǎn)的任一側(cè)，則水解速率已顯示較慢。

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胰蛋白酶相關(guān)研究：

細(xì)胞分離優(yōu)化系統(tǒng)

胰凝乳蛋白酶

膠原酶

脫氧核糖核酸酶?I

內(nèi)切蛋白酶Lys-C

肝細(xì)胞分離系統(tǒng)

透明質(zhì)酸酶

新生兒心肌細(xì)胞分離系統(tǒng)

中性蛋白酶（分散酶）

木瓜蛋白酶解離系統(tǒng)

蛋白酶K

胰蛋白酶抑制劑