重組蛋白——囊泡型谷氨酸轉(zhuǎn)運(yùn)蛋白1VGLUT1
囊泡谷氨酸轉(zhuǎn)運(yùn)體（VGLUTs）包括3個(gè)成員，VGLUT1、VGLUT2和VGLUT3。囊泡谷氨酸轉(zhuǎn)運(yùn)體（VGLUTs）的異常會(huì)導(dǎo)致興奮性神經(jīng)遞質(zhì)谷氨酸的異常，從而誘發(fā)多種神經(jīng)系統(tǒng)疾病。
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囊泡型谷氨酸轉(zhuǎn)運(yùn)蛋白1VGLUT1，又稱BNPI和SLc17a7，zui初被鑒定為腦特異性磷酸轉(zhuǎn)運(yùn)蛋白。與相關(guān)的VGLUT-2一樣，VGLUT-1對(duì)谷氨酸的攝取和儲(chǔ)存既是必要的，也是充分的，因此構(gòu)成了谷氨酸能表型的決定因素。這兩種VGLUT都不同于血漿膜轉(zhuǎn)運(yùn)蛋白，因?yàn)樗鼈兪怯煽缒遗菽さ馁|(zhì)子電化學(xué)梯度驅(qū)動(dòng)的。
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VGLUT 1和VGLUT 2呈現(xiàn)互補(bǔ)表達(dá)模式?？偠灾?，它們是目前谷氨酸能神經(jīng)末梢和谷氨酸能突觸的zui好標(biāo)記物。
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VGLUT 1 - 135 302，這個(gè)來(lái)自德國(guó)SySy的囊泡谷氨酸轉(zhuǎn)運(yùn)體1（抗體），特異性針對(duì)VGLUT1蛋白（經(jīng)過(guò)敲除驗(yàn)證K.O），推薦作為谷氨酸能神經(jīng)末梢的標(biāo)記物。（使用說(shuō)明：VGLUT 1在煮沸后聚集，因此建議對(duì)未煮沸的樣品進(jìn)行SDS-PAGE分析。）
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發(fā)表文章超100+：Nature, Cell，課代表~
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WB（23+）：
Vesicular Glutamate Transporters (SLCA17 A6, 7, 8) Control Synaptic Phosphate Levels.
Cheret C, Ganzella M, Preobraschenski J, Jahn R, Ahnert-Hilger G
Cell reports (2021) 342: 108623. 135 302 WB, ICC; tested species: human,mouse
IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.
Tanabe Y, Naito Y, Vasuta C, Lee AK, Soumounou Y, Linhoff MW, Takahashi H
Nature communications (2017) 81: 408. 135 302 WB
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IP（2+）：
Regulation of the Hippocampal Network by VGLUT3-Positive CCK- GABAergic Basket Cells.
Fasano C, rocchetti J, Pietrajtis K, Zander JF, Manseau F, Sakae DY, Marcus-Sells M, Ramet L, Morel LJ, Carrel D, Dumas S, et al.
Frontiers in cellular neuroscience (2017) 11: 140. 135 302 WB, IP; tested species: mouse
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ICC（33+）：
Neuronal defects in a human cellular model of 22q11.2 deletion syndrome.
Khan TA, Revah O, Gordon A, Yoon SJ, Krawisz AK, Goold C, Sun Y, Kim CH, Tian Y, Li MY, Schaepe JM, et al.
Nature medicine (2020) : . 135 302 ICC; tested species: human
Direct pericyte-to-neuron reprogramming via unfolding of a neural stem cell-like program.
Karow M, Camp JG, Falk S, Gerber T, Pataskar A, Gac-Santel M, Kageyama J, Brazovskaja A, Garding A, Fan W, Riedemann T, et al.
Nature neuroscience (2018) : . 135 302 ICC; tested species: human
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IHC（40+）：
Context-Specific Striatal Astrocyte Molecular Responses Are Phenotypically Exploitable.
Yu X, Nagai J, Marti-Solano M, Soto JS, Coppola G, Babu MM, Khakh BS
Neuron (2020) : . 135 302 IHC; tested species: mouse
Hyperactivity with Disrupted Attention by Activation of an Astrocyte Synaptogenic Cue.
Nagai J, Rajbhandari AK, Gangwani MR, Hachisuka A, Coppola G, Masmanidis SC, Fanselow MS, Khakh BS
Cell (2019) : . 135 302 IHC; tested species: mouse
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Other（1+）：
Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae.
Ishikawa M, Ohnishi H, Skerleva D, Sakamoto T, Yamamoto N, Hotta A, Ito J, Nakagawa T
Journal of tissue engineering and regenerative medicine (2017) 116: 1766-1778. 135 302
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部分結(jié)果圖展示：
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文章來(lái)源：https://www.amyjet.com/featured/vglut1.shtml
