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Mag-Fura-2,細(xì)胞內(nèi)鎂熒光探針

2022-10-10 09:57 作者:BIOFOUNT范德生物  | 我要投稿

Mag-fura-2 四鉀鹽是一種細(xì)胞內(nèi)鎂離子特異性熒光探針,屬于紫外光激發(fā)的比率式探針。水溶性的鹽形式適用于通過(guò)顯微注射、從膜片移液管注入。


Mag-fura-2 四鉀鹽是一種細(xì)胞內(nèi)鎂離子特異性熒光探針,屬于紫外光激發(fā)的比率式探針。水溶性的鹽形式適用于通過(guò)顯微注射、從膜片移液管注入。

溶解度:Solubility Soluble in water

性狀:Solid power

儲(chǔ)藏條件:torage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)


案例:

The effects of Mrs2 KD on the intracellular Mg2+ homeostasis.: The images of HeLa cells simultaneously loaded with both Mag-Fura-2 (a) and MitoFluor Red (b). (c–f) The enlarged view of the boxed area in panel a and b. (c,d) The localization of Mag-Fura-2 in the same cell shown in the panel a and b before (c) and after (d) cell membrane permeabilization by digitonin. (e) Localization of MitoFluor Red after cell membrane permeabilization by digitonin. (f) The merged image were shown. (g) The mitochondrial Mg2+ level in control and Mrs2 KD HeLa cells. Comparing the ratio of Mag-Fura-2 fluorescence excited at 340?nm to at 380?nm, indicating mitochondrial Mg2+ level, revealed a decrease in Mrs2 KD cells (n?=?148 for the control and n?=?105 for Mrs2 KD cells from 5 experiments). (h–j) The images of HeLa cells normally loaded with Mag-Fura-2. (h) Fluorescence image of Mrs2 KD cells labeled with emGFP. (i) The merged fluorescence images of control and emGFP- labeled (red) Mrs2 KD cells loaded with Mag-Fura-2 (green). (j) The fluorescence emission ratio image at excitation of 340 and 380?nm of HeLa cells loaded with Mag-Fura-2. Pseudo color showed the emission ratio of Mag-Fura-2 signals excited at 340?nm to 380?nm. (k) The cytosolic Mg2+ level in control and Mrs2 KD cells. In Mrs2 KD cells, cytosolic Mg2+ level is higher than that in control cells (n?=?304 for the control and n?=?384 Mrs2 KD cells from 5 experiments).



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