Product Details

The 10F.9G2 monoclonal antibody reacts with mouse PD-L1 (programmed death ligand 1) also known as B7-H1 or CD274. PD-L1 is a 40 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNγ stimulated monocytes, epithelial cells and endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors and results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and PD-1. The 10F.9G2 antibody has been shown to block the interaction between PD-L1 and PD-1 and between PD-L1 and B7-1 (CD80).

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BioXCell?艾美捷BE0101體內(nèi)單克隆抗小鼠PD-L1(B7-H1)抗體:

10F.9G2單克隆抗體與小鼠PD-L1（程序性死亡配體1）反應(yīng)，也稱為B7-H1或CD274。PD-L1是一種40 kDa的I型跨膜蛋白，屬于Ig超家族的B7家族。PD-L1在T淋巴細(xì)胞，B淋巴細(xì)胞，NK細(xì)胞，樹突狀細(xì)胞以及IFNγ刺激的單核細(xì)胞，上皮細(xì)胞和內(nèi)皮細(xì)胞上表達(dá)。PD-L1與其受體PD-1結(jié)合，PD-4存在于CD8和CD1胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和骨髓細(xì)胞上。

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BioXCell艾美捷體內(nèi)單克隆抗小鼠PD-L1(B7-H1)抗體規(guī)格：

# ?BXC-BE0101

Isotype?Rat IgG2b, κ

Recommended Isotype Control(s)?InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin

Recommended Dilution Buffer?InVivoPure pH 6.5 Dilution Buffer

Immunogen?Mouse CD274

Reported Applications?in vivo PD-L1 blockade

Immunofluorescence

Immunohistochemistry (frozen)

Flow cytometry

Western blot

Formulation?PBS, pH 6.5

Contains no stabilizers or preservatives

Endotoxin?<2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity?>95%

Determined by SDS-PAGE

Sterility?0.2 μM filtered

Production?Purified from tissue culture supernatant in an animal free facility

Purification?Protein G

RRID?AB_10949073

Molecular Weight?150 kDa

Storage?The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

同種型?大鼠IgG2b， κ

推薦的同種型對照?體內(nèi)MAb大鼠IgG2b同種型對照，抗鎖孔帽貝血藍(lán)蛋白

推薦的稀釋緩沖液?體內(nèi)純?pH 6.5 稀釋緩沖液

免疫原?鼠標(biāo)?CD274

報(bào)告的應(yīng)用程序?體內(nèi)PD-L1阻斷

免疫熒光

免疫組化（冷凍）

流式細(xì)胞術(shù)

蛋白質(zhì)印跡

配方?PBS，pH 6.5

不含穩(wěn)定劑或防腐劑

內(nèi)毒素?<2EU/mg （<0.002EU/μg）

通過LAL凝膠凝血測定法測定

純度?>95%

由?SDS-PAGE 決定

無?菌?0.2 μM 過濾

生產(chǎn)?從無動(dòng)物設(shè)施中的組織培養(yǎng)上清液中純化

純化?蛋白?G

里德?AB_10949073

分子量?150 千達(dá)

存儲(chǔ)?抗體溶液應(yīng)以4°C的儲(chǔ)備濃度儲(chǔ)存。 不要凍結(jié)。